File:CRISPR-Cas9 mode of action.png
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CRISPR-Cas9_mode_of_action.png (717 × 197 pixels, file size: 32 KB, MIME type: image/png)
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DescriptionCRISPR-Cas9 mode of action.png |
English: A duplex of crRNA and tracrRNA acts as guide RNA to introduce a specifically located gene modification based on the RNA 5’ upstream of the crRNA. Cas9 binds the tracrsRNA and needs a DNA binding sequence (5’NGG3’), which is called protospacer adjacent motif (PAM). After binding, Cas9 introduces a DNA double strand break, which is then followed by gene modification via homologous recombination (HDR) or non-homologous end joining (NHEJ). |
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Source | Own work |
Author | ViktoriaAnselm |
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:32, 18 December 2015 | 717 × 197 (32 KB) | ViktoriaAnselm (talk | contribs) | User created page with UploadWizard |
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18 December 2015
image/png
30845067730bf48d78ea9bcad1b4bcd7fe4f1098
32,335 byte
197 pixel
717 pixel
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