File:41396 2019 362 Fig1 HTML.webp
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English: Zobellviridae of order Caudovirales and their Lentibacter hosts. a. Enrichment of phages specific for Lentibacter sp. SH36 from North Sea water. The experimental setup consists of: (i) Phage enrichments (blue lines) – logarithmic phase cells added to nutrient amended, 0.2 µm filtered seawater, (ii) Positive control for cell growth (green line) – logarithmic phase cells added to artificial seawater and (iii) Negative control (orange line) – only nutrient amended, 0.2 µm filtered seawater, no cells added. In the S1 and S2 enrichments the decrease in OD at 18 h is most likely due to phage cell lysis. No growth was detected in the negative control. b. Transmission electron micrograph of molybdenum stained, cell debris bound Lentibacter virus vB_LenP_ICBM1 (genus Siovirus) and uranyl acetate stained, free Lentibacter virus vB_LenP_ICBM2 (genus Veravirus) . Scale bar: 100 nm. c. Agarose gel electrophoresis of cellular DNA from i) Lentibacter sp. SH36 phage infected cells, S2 enrichment (lane 1, blue arrow) and ii) not infected cells, positive control for cell growth (lane 2, green arrow). Blue circle: intracellular phage DNA. Lane 3: 1 kbp Plus DNA Ladder |
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Fig. 1 at https://www.nature.com/articles/s41396-019-0362-7 Cobaviruses – a new globally distributed phage group infecting Rhodobacteraceae in marine ecosystems. In: ISME Journal 13, 1404–1421. doi:10.1038/s41396-019-0362-7  |
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| Author | Vera Bischoff, Boyke Bunk, Jan P. Meier-Kolthoff, Cathrin Spröer, Anja Poehlein, Marco Dogs, Mary Nguyen, Jörn Petersen, Rolf Daniel, Jörg Overmann, Markus Göker, Meinhard Simon, Thorsten Brinkhoff, Cristina Moraru | ||
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 07:16, 29 June 2021 | | 1,911 × 734 (99 KB) | Ernsts (talk | contribs) | Uploaded a work by Vera Bischoff, Boyke Bunk, Jan P. Meier-Kolthoff, Cathrin Spröer, Anja Poehlein, Marco Dogs, Mary Nguyen, Jörn Petersen, Rolf Daniel, Jörg Overmann, Markus Göker, Meinhard Simon, Thorsten Brinkhoff, Cristina Moraru from Fig. 1 at https://www.nature.com/articles/s41396-019-0362-7 Cobaviruses – a new globally distributed phage group infecting ''Rhodobacteraceae'' in marine ecosystems. In: ISME Journal 13, 1404–1421 with UploadWizard |
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