File:Fpls-02-00036-g001.png

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English: Kinase reactions showing substrate specificity differences between CPK16 compared to CPK1, 10, and 34. (A) Left side: Coomassie Brilliant Blue-stained gels showing relative loading amounts of substrates for each kinase reaction. (B) Right side: Autoradiogram of stained gel showing proteins labeled by 32P-phosphorylation. V: GST vector only (ps627), NR: nitrate reductase peptide-GFP fusion (G-GFP-NR-ST, ps699); S > A: S109A substitution in G-GFP-S109-ST (ps1256; mutant = DVLKSEQKEMAYREDPY); wt: G-GFP-S109-ST (ps1160; wild type = DVLKSEQKEMSYREDPY); Di19-2: Di19-2 full-length GST-fusion protein (G-Di19-2, ps1195). Results shown are representative of multiple independent analyses.
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Source doi:10.3389/fpls.2011.00036
Author Amy Curran, Ing-Feng Chang, Chia-Lun Chang, Shilpi Garg, Rodriguez Milla Miguel, Yoshimi D. Barron, Ying Li, Shawn Romanowsky, John C. Cushman, Michael Gribskov, Alice C. Harmon, Jeffrey F. Harper

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