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English: Principal component analysis and clustering of crude cell wall sugar estimates for the selected mutants. (A) GC–MS analysis of sugar alditols of crude cell wall material for indicated mutants. (B) Principal component analysis (PCA) of the mol% sugar values from the crude cell wall material. The first two components explain 93.12% of the variation. The loadings for the variables are displayed in supplementary Figure S3 in Supplementary Material. The ellipses indicate clustering of mutant sugar profiles based on the k-mediod algorithm. The number of clusters was estimated based on the silhouette width, i.e., the dissimilarity in sugar profiles as measured by the Euclidean distances between the profiles. Wild-type (black), and mutants affecting cellulose (irx5; red), xylan (irx9; blue), and potentially lignin (irx12; orange) are indicated in different colors.