File:Fert5.JPG

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Description bovine fertilization
Date 9/11/08
Source Own work
Author Mtengows

Cytochalasin-treated zona pellucida-free oocytes exposed to acrosome-reacted bull sperm display markedly different incorporation. Cytochalasin treatment alters the morphology of the oocyte microvilli, producing areas devoid of microvilli and microvilli with short, flat, club-like processes. After either 30 min (A), 60 min (B), 120 min (C), or 240 min (D) of sperm exposure with the oocytes in the presence of cytochalasin, the remaining oocyte microvilli are seen contacting the sperm head in various locations, yet the posterior sperm head, implantation fossa, and sperm tail with mitochondria remain outside the ooplasm, suggesting that oocyte microfilament assembly is important for successful sperm entry in the bovine system. Images originally published in: Tengowski, MW (2004) Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development. Methods in Molecular Biology (Clifton, N.J.), 253, 165-99.

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current22:20, 11 September 2008Thumbnail for version as of 22:20, 11 September 2008577 × 575 (104 KB)Mtengows (talk | contribs){{Information |Description=bovine fertilization |Source=self-made |Date=9/11/08 |Author= Mtengows |Permission= |other_versions= }} Cytochalasin-treated zona pellucida-free oocytes exposed to acrosome-reacted bull sperm display markedly d

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