File:DNA Nanoball Adapter Ligation.png
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DescriptionDNA Nanoball Adapter Ligation.png |
English: The Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligated, the DNA is circularized and a restriction enzyme cleaves the DNA. AD3L and AD3R are added, the DNA is circularized, and a type III restriction enzyme is added. This cleaves out a 300 - 400bp piece of DNA and AD4 replaces it to complete the circular, adapter ligated template DNA. |
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Source | Own work | |||
Author | Suspencewl | |||
Permission (Reusing this file) |
I, the copyright holder of this work, hereby publish it under the following license:
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:51, 28 February 2011 | 902 × 1,026 (542 KB) | Suspencewl (talk | contribs) | Lower resolution so it can fit in page better as non-thumb | |
22:58, 24 February 2011 | 1,282 × 1,026 (332 KB) | Suspencewl (talk | contribs) | Reverted to version as of 22:46, 24 February 2011 | ||
22:49, 24 February 2011 | 1,202 × 1,026 (660 KB) | Suspencewl (talk | contribs) | The Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligated, the DNA is circularized and a restrict | ||
22:46, 24 February 2011 | 1,282 × 1,026 (332 KB) | Suspencewl (talk | contribs) | {{Information |Description ={{en|1=The Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligate |
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