File:Confocal analysis of dermal fibroblasts after heat shock stress (progeria).jpg
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English: Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours at 37°C. Cells were processed for indirect immunofluorescence labelling using anti-lamin A/C antibodies (panel A, a to f), anti-lamin B1 antibodies (panel B, g to r) and anti-vimentin antibodies (panel B j to i and p to r). Control and HGPS fibroblasts are indicated. (A) Fibroblasts were immunostained with anti-lamin A/C antibodies. Note the increased number of dysmorphic nuclei in HGPS fibroblasts compared to control 24 hours after recovery from heat shock. (B) Fibroblasts were immunostained with anti-lamin B1 and vimentin at times indicated after heat shock. Bar, 10 μm. Paradisi et al. BMC Cell Biology 2005 6:27 doi:10.1186/1471-2121-6-27 |
| Date | |
| Source | Dermal fibroblasts in Hutchinson-Gilford progeria syndrome with the lamin A G608G mutation have dysmorphic nuclei and are hypersensitive to heat stress |
| Author | Mauro Paradisi , Dayle McClintock , Revekka L Boguslavsky , Christina Pedicelli1 , Howard J Worman and Karima Djabali |
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© 2005 Paradisi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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| current | 15:52, 5 January 2010 | | 900 × 1,347 (273 KB) | CopperKettle (talk | contribs) | {{Information |Description={{en|1=Confocal analysis of dermal fibroblasts after heat shock stress. Cells were incubated at 45°C for 30 minutes, then either immediately fixed (time 0) or allowed to recover for 24 or 48 hours at 37°C. Cells were processed |
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