File:A-dynamin-1--dynamin-3--and-clathrin-independent-pathway-of-synaptic-vesicle-recycling-mediated-by-elife01621f010.jpg

English: Neuronal cultures of the indicated genotypes were stimulated with high K+ for 90 s and processed by high pressure-freezing/freeze-substitution EM. The diameter of all vesicles (within the 20–200 nm range) in sections of individual nerve terminals before stimulation and after 3 and/or 10 min recovery were analyzed. (A) WT and Dyn1 KO nerve terminals. (B) Dyn3 KO and Dyn1/3 DKO nerve terminals. Diameters were binned at 10 nm intervals and the average diameters (± SD) of vesicles smaller than 80 nm (i.e., vesicles considered as SVs) are indicated in each field. Note (a) the shift of the peak of SV diameter from the 30 to the 40 nm bin, (b) the larger average SV size and (c) the larger SD during the recovery after the stimulus in all genotypes. Nerve terminals analyzed: 21 for WT R, 106 for WT 3 min, 18 for Dyn1 KO R, 123 for Dyn1 KO 3 min, 20 for Dyn3 KO R, 86 for Dyn3 KO 3 min, 27 for Dyn3 KO 10 min, 17 for Dyn1/3 DKO R, 114 for Dyn1/3 DKO 3 min, 42 for Dyn1/3 DKO 10 min.